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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
08/06/2021 |
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Actualizado : |
02/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GASTAL, G.D.A.; SCARLET D.; MELCHERT M.; ERTL R.; AURICH, C. |
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Afiliación : |
GUSTAVO DESIRE ANTUNES GASTAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./ Center for Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.; DRAGOS SCARLET, Division of Obstetrics, Gynecology and Andrology, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria;; MARIA MELCHERT, Center for Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.; REINHARD ERT, Vetcore Facility, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.; CHRISTINE AURICH, Center for Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria. |
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Título : |
Epigenetic changes in equine embryos after short-term storage at different temperaturas. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Animals, 5 May 2021, Volume 11, Issue 5, Article number 1325. Open Access. Doi: https://doi.org/10.3390/ani11051325 |
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DOI : |
10.3390/ani11051325 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 16 March 2021/Accepted: 3 May 2021/Published: 6 May 2021. This work was supported by University of Veterinary Medicine Vienna, Austria. |
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Contenido : |
Simple Summary: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern.
The aim of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, concerning morphological development, expression of candidate genes associated with embryo growth and
development, maternal recognition of pregnancy, methylation, apoptosis and gene-specific and global DNA methylation. Temperature during storage did not affect embryo size. There were no changes in pH and lipid peroxidation of the medium irrespective of group. mRNA expression and gene-specific DNA methylation of genes related to growth and development, maternal recognition of pregnancy, DNA methylation and apoptosis in stored embryos (5 ?C and 20 ?C) were altered when compared to fresh embryos. Therefore, our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Short-term storage, regardless of temperature, may compromise embryo development after transfer.
Abstract: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and the establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern. The aim
of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, on: (i) morphological development; (ii) expression of candidate genes associated with embryo growth and development,
maternal recognition of pregnancy, methylation and apoptosis, and (iii) gene-specific and global DNA methylation. Embryos (n = 80) were collected on day seven or day eight after ovulation and assigned to four groups: day seven control (E7F, fresh); day seven, stored for 24 h at 5 ?C (E5C);
day seven, stored for 24 h at 20 ?C (E20C) and day eight control (E8F, fresh 24h time control). The embryos and the storage medium (EquiHold, holding medium, Minitube, Tiefenbach, Germany) from all treatment groups were analyzed for (i) medium temperature, pH, and lipid peroxidation
(malondialdehyde; MDA) and (ii) embryo morphology, mRNA expression and DNA methylation (immunohistochemistry and gene-specific DNA methylation). The size of embryos stored at 5 ?C was larger (p < 0.01), whereas embryos stored at 20 ?C were smaller (p < 0.05) after 24 h. There
were no changes in pH and MDA accumulation irrespective of the group. The mRNA expression of specific genes related to growth and development (POU5F1, SOX2, NANOG), maternal recognition of pregnancy (CYP19A1, PTGES2), DNA methylation (DNMT1, DNMT3A, DNMT3B) and apoptosis
(BAX) in the E5C and E20C were either up or downregulated (p < 0.05) when compared to controls (E7F and E8F). The immune expression of 5mC and 5hmC was similar among treatment groups. Percentage of methylation in the CpG islands was lower in the specific genes ESR1, NANOG and
DNMT1 (p < 0.001) in E20C embryos when compared to E8F (advanced embryo stage). Therefore,our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Although our results suggest
some beneficial effects of storage at 20 ?C in comparison to 5 ?C, the short-term storage, regardless of temperature, modified gene expression and methylation of genes involved in embryo development and may compromise embryo viability and development after transfer. MenosSimple Summary: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern.
The aim of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, concerning morphological development, expression of candidate genes associated with embryo growth and
development, maternal recognition of pregnancy, methylation, apoptosis and gene-specific and global DNA methylation. Temperature during storage did not affect embryo size. There were no changes in pH and lipid peroxidation of the medium irrespective of group. mRNA expression and gene-specific DNA methylation of genes related to growth and development, maternal recognition of pregnancy, DNA methylation and apoptosis in stored embryos (5 ?C and 20 ?C) were altered when compared to fresh embryos. Therefore, our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Short-term storage, regardless of temperature, may compromise embryo development after transfer.
Abstract: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and... Presentar Todo |
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Palabras claves : |
Development; Embryo; Embryo-maternal recognition; Equine; Methylation; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; Transport. |
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Thesagro : |
EQUINOS. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16671/1/animals-11-01325.pdf
https://www.mdpi.com/2076-2615/11/5/1325/pdf
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Marc : |
LEADER 04973naa a2200301 a 4500
001 1062116
005 2022-09-02
008 2021 bl uuuu u00u1 u #d
024 7 $a10.3390/ani11051325$2DOI
100 1 $aGASTAL, G.D.A.
245 $aEpigenetic changes in equine embryos after short-term storage at different temperaturas.$h[electronic resource]
260 $c2021
500 $aArticle history: Received: 16 March 2021/Accepted: 3 May 2021/Published: 6 May 2021. This work was supported by University of Veterinary Medicine Vienna, Austria.
520 $aSimple Summary: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern. The aim of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, concerning morphological development, expression of candidate genes associated with embryo growth and development, maternal recognition of pregnancy, methylation, apoptosis and gene-specific and global DNA methylation. Temperature during storage did not affect embryo size. There were no changes in pH and lipid peroxidation of the medium irrespective of group. mRNA expression and gene-specific DNA methylation of genes related to growth and development, maternal recognition of pregnancy, DNA methylation and apoptosis in stored embryos (5 ?C and 20 ?C) were altered when compared to fresh embryos. Therefore, our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Short-term storage, regardless of temperature, may compromise embryo development after transfer. Abstract: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and the establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern. The aim of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, on: (i) morphological development; (ii) expression of candidate genes associated with embryo growth and development, maternal recognition of pregnancy, methylation and apoptosis, and (iii) gene-specific and global DNA methylation. Embryos (n = 80) were collected on day seven or day eight after ovulation and assigned to four groups: day seven control (E7F, fresh); day seven, stored for 24 h at 5 ?C (E5C); day seven, stored for 24 h at 20 ?C (E20C) and day eight control (E8F, fresh 24h time control). The embryos and the storage medium (EquiHold, holding medium, Minitube, Tiefenbach, Germany) from all treatment groups were analyzed for (i) medium temperature, pH, and lipid peroxidation (malondialdehyde; MDA) and (ii) embryo morphology, mRNA expression and DNA methylation (immunohistochemistry and gene-specific DNA methylation). The size of embryos stored at 5 ?C was larger (p < 0.01), whereas embryos stored at 20 ?C were smaller (p < 0.05) after 24 h. There were no changes in pH and MDA accumulation irrespective of the group. The mRNA expression of specific genes related to growth and development (POU5F1, SOX2, NANOG), maternal recognition of pregnancy (CYP19A1, PTGES2), DNA methylation (DNMT1, DNMT3A, DNMT3B) and apoptosis (BAX) in the E5C and E20C were either up or downregulated (p < 0.05) when compared to controls (E7F and E8F). The immune expression of 5mC and 5hmC was similar among treatment groups. Percentage of methylation in the CpG islands was lower in the specific genes ESR1, NANOG and DNMT1 (p < 0.001) in E20C embryos when compared to E8F (advanced embryo stage). Therefore,our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Although our results suggest some beneficial effects of storage at 20 ?C in comparison to 5 ?C, the short-term storage, regardless of temperature, modified gene expression and methylation of genes involved in embryo development and may compromise embryo viability and development after transfer.
650 $aEQUINOS
653 $aDevelopment
653 $aEmbryo
653 $aEmbryo-maternal recognition
653 $aEquine
653 $aMethylation
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aTransport
700 1 $aSCARLET D.
700 1 $aMELCHERT M.
700 1 $aERTL R.
700 1 $aAURICH, C.
773 $tAnimals, 5 May 2021, Volume 11, Issue 5, Article number 1325. Open Access. Doi: https://doi.org/10.3390/ani11051325
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Las Brujas. |
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Fecha actual : |
05/04/2020 |
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Actualizado : |
06/10/2020 |
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Tipo de producción científica : |
Artículos en Revistas Agropecuarias |
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Autor : |
NERVI, E.; SCHINCA, C.; CARBAJALES, A.; DE GUIDA, M.; PIPPOLO, D.; DABEZIES, M.; DEMARÍA, K.; OLIVERA, A.; MARTÍNEZ, M.; BOCCARDI, L.; MIGUEZ, D.; VILLANUEVA, J. P.; PARDO, H.; LAMELA, S.; PECOITS, E.; CABEZAS, A.; MOIZO, P.; CAFARO, N.; CARRASCO-LETELIER, L. |
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Afiliación : |
ELIANA NERVI, Centro Tecnológico del Agua (CTAgua); CECILIA SCHINCA, Centro Tecnológico del Agua (CTAgua); ALEJANDRO CARBAJALES, Centro Tecnológico del Agua (CTAgua); MARIELA DE GUIDA, Laboratorio Tecnológico del Uruguay (LATU); DANIEL PIPPOLO, Laboratorio Tecnológico del Uruguay (LATU); MARÍA DABEZIES, Laboratorio Tecnológico del Uruguay (LATU); KARINA DEMARÍA, Laboratorio Tecnológico del Uruguay (LATU); ANDRÉS OLIVERA, Laboratorio Tecnológico del Uruguay (LATU); MÓNICA MARTÍNEZ, Laboratorio Tecnológico del Uruguay (LATU); LUCÍA BOCCARDI, Latitud - Fundación LATU, Uruguay; DIANA MIGUEZ, Latitud - Fundación LATU, Uruguay; JUAN PABLO VILLANUEVA, Parque Científico Tecnológico de Pando (PCTP); HELENA PARDO, Parque Científico Tecnológico de Pando (PCTP); SILVIA LAMELA, Universidad de Montevideo (UM); ERNESTO PECOITS, Universidad Tecnológica (UTEC); ANGELA CABEZAS, Universidad Tecnológica (UTEC); PAUL MOIZO, Gestión Compartida de la Cuenca del Río Santa Lucía (GCSL); NORBERTO CAFARO, Efice S.A; LEONIDAS CARRASCO-LETELIER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Hacia el uso sostenible del agua en la producción. Proyecto Custodia del Agua en la Cuenca del Santa Lucía. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Revista INIA Uruguay, 2020, no. 60, p. 75-78. |
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Serie : |
(Revista INIA; 60). |
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ISSN : |
1510-9011 |
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Idioma : |
Español |
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Contenido : |
El concepto de custodia del agua surge como una respuesta a problemas comunes en el uso responsable y sostenible del agua dulce y propone esfuerzos colectivos con origen en iniciativas individuales, voluntarias y colaborativas para gestionarla de manera sostenible. |
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Palabras claves : |
Centro Tecnológico del Agua (CTAgua); CUENCA DE SANTA LUCÍA. |
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Thesagro : |
AGUA. |
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Asunto categoría : |
P01 Conservación de la naturaleza y recursos de La tierra |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14377/1/Rev-INIA-60-Marzo-2020-p-75-78.pdf
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Marc : |
LEADER 01360naa a2200397 a 4500
001 1061005
005 2020-10-06
008 2020 bl uuuu u00u1 u #d
022 $a1510-9011
100 1 $aNERVI, E.
245 $aHacia el uso sostenible del agua en la producción. Proyecto Custodia del Agua en la Cuenca del Santa Lucía.$h[electronic resource]
260 $c2020
490 $a(Revista INIA; 60).
520 $aEl concepto de custodia del agua surge como una respuesta a problemas comunes en el uso responsable y sostenible del agua dulce y propone esfuerzos colectivos con origen en iniciativas individuales, voluntarias y colaborativas para gestionarla de manera sostenible.
650 $aAGUA
653 $aCentro Tecnológico del Agua (CTAgua)
653 $aCUENCA DE SANTA LUCÍA
700 1 $aSCHINCA, C.
700 1 $aCARBAJALES, A.
700 1 $aDE GUIDA, M.
700 1 $aPIPPOLO, D.
700 1 $aDABEZIES, M.
700 1 $aDEMARÍA, K.
700 1 $aOLIVERA, A.
700 1 $aMARTÍNEZ, M.
700 1 $aBOCCARDI, L.
700 1 $aMIGUEZ, D.
700 1 $aVILLANUEVA, J. P.
700 1 $aPARDO, H.
700 1 $aLAMELA, S.
700 1 $aPECOITS, E.
700 1 $aCABEZAS, A.
700 1 $aMOIZO, P.
700 1 $aCAFARO, N.
700 1 $aCARRASCO-LETELIER, L.
773 $tRevista INIA Uruguay, 2020, no. 60, p. 75-78.
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INIA Las Brujas (LB) |
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